|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
pax6
|
|
laevis
|
NF stage 21
|
neural tube
,
optic field
|
otx2
|
|
laevis
|
NF stage 25
|
olfactory placode
,
brain
,
forebrain
,
optic field
|
myocd
|
|
laevis
|
NF stage 41
|
heart
,
cardiac mesoderm
|
ap2a2
|
|
laevis
|
NF stage 33 and 34
|
head region
,
neural crest
,
anterior branchial crest
,
posterior branchial crest
|
foxd3
|
|
laevis
|
NF stage 23
|
neural crest
,
cranial neural crest
|
tbx20
|
|
laevis
|
NF stage 41
|
heart
,
cardiac mesoderm
|
nkx2-5
|
|
laevis
|
NF stage 41
|
heart
,
cardiac mesoderm
|
myh6
|
|
laevis
|
NF stage 41
|
heart
,
cardiac mesoderm
|
|
|
Fig. 6. FoxO4MO injection affects eye, heart and cranial crest development. (A) FoxO4 depletion results in lack of eye structures. Head of an unilaterally FoxO4MO injected embryo (40 ng/blastomere) at stage 39 (lateral view). The eye is almost absent. Middle: Pax-6 whole mount in situ hybridization of control and unilaterally FoxO4MO injected embryos at stage 21. Bottom: Otx-2 whole mount in situ hybridization of control and unilaterally FoxO4MO injected embryos at stage 25. (B) FoxO4 depletion affects cranial crest and jaw development. In situ hybridization of unilaterally FoxO4MO injected embryos with the neural crest markers FoxD3 at stage 23 and AP2α at stage 33. Red arrows indicate the lack of FoxD3 and AP2α expression. Bottom: Alcian blue stained control and unilaterally FoxO4MO injected embryo (30 ng/blastomere) at stage 47. (C) FoxO4 depletion results in failure of heart development. Top: lateral view on control and bilaterally FoxO4MO injected embryo at stage 41. Right: control heart of stage 43 embryo and heart of FoxO4MO injected embryo (20 ng/blastomere at 2-cell stage). Bottom: control- and unilaterally FoxO4Mo injected embryos. myocd and MHCα in situ hybridization reveals reduced expression at the injected side. In situ hybridization of unilaterally FoxO4MO injected embryos with Nkx2.5, GATA-5 and Tbx20. Red asterisks indicate the side of injection. ba, branchial arches; c, conus; ch, ceratohyale; mc, Meckel's cartilage; v, ventricle. Percentages of phenotypes are given in supplementary Table S7. |