| |
Fig. 6. GATA-4 is an early marker for the developing heart.
Embryos were analyzed by whole-mount in situ hybridization for
GATA-4 RNA. Stages are as follows: 18 (A,B); 26 (C,D); 34
(E,F); 40 (G,H). The photographs shown in B,D,F and H are high
magnification views of the embryos shown in A,C,E and G,
respectively. Arrows indicate detection of GATA-4 message by
alkaline phosphatase staining in the region of the developing
heart; non-specific staining around the residual blastocoel (for
example, in A) is seen with control sense-strand probes. Note that
by stage 40, GATA-4 message is detected in other organs where
transcription is high in adults, such as the stomach. In all cases,
dorsal is towards the top. In C and D, anterior is to the left,
otherwise anterior is to the right. Large arrows in F and H indicate
staining in the developing heart (h). For reference, cg indicates the
position of the cement gland. |
