| |
Fig. 1. Expression of es1:EGFP and endogenous es1 in embryos. (A) Structure of Xenopus laevis es1 gene (upper) and the construct used for transgenesis (lower). Open squares represent exons. pA, poly adenylation signals. (B-E) Representative images of es1: egfp transgenic embryos. C, D and E show EGFP fluorescence images. B shows a normal light image of the same field of C. Asterisks in B and C show an EGFP-negative embryo. Arrowheads in D indicate cells expressing EGFP in dorsal midline. Arrowheads in E indicate EGFP expression in oral primodium, pharyngeal arches and anus region. (F, G) Representative images of WISH showing endogenous es1 expression in embryos. Arrowheads in G indicate es1 expression in oral primordium, pharyngeal arches and anus region. B, C, D and F are dorsal images. Embryos in E and G are anterior left and dorsal up. Embryonic stages are indicated. Bars, 500 µm. |
