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Fig. 1. Localization of endogenous VgJ
mRNA in cultured oocytes. Oocytes were
grown in Leibowitz medium supplemented
with vitellogenin-containing frog serum as
previously described (Yisraeli and Melton,
1988). After the indicated time in culture,
the oocytes were fixed and hybridized with
a Vgl probe made to the coding region
(Melton, 1987; Yisraeli and Melton, 1988).
In these dark-field photographs, silver
autoradiographic grains appear white. |
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Fig. 4. Effect of cytoskeletal inhibitors on Vgl mRNA in
late-stage oocytes. Stage V/VI oocytes were incubated in
saline (untreated), cytochalasin B (25ug/mL, cvtochalasin
B), or nocodazole (10ug/mL, nocodazole) overnight at
20°C and then analyzed by in situ hybridization for the
localization of Vgl mRNA. In addition, detergent extracts
of the oocytes were performed for each treatment and the
corresponding Northern blots are shown below each
section, p, pellet; s, soluble fraction. |
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| |
Fig. 5. Effect of cytoskeletal inhibitors on Vgl mRNA in
middle-stage oocytes. Late stage III oocytes were cultured
for 5 days in the presence of medium and serum alone
(+serum), medium and serum with cytochalasin B (+serum
+cytochalasin 6), and medium and serum with nocodazole
(+serum +nocodazole). In situ hybridization visualized the
location of the Vgl mRNA in the oocyte sections. At left is
a section from an oocyte cultured in medium alone for 5
days, showing no localization whatsoever (-serum). |
