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Figure 4.
Colocalization of VgRBP60 Protein and Vg1 RNA
(A and B) The distribution of VgRBP60 within stage III oocytes was determined by immunocytochemistry using anti-peptide antibody directed against PTB/hnRNP I (courtesy of D. Black). Optical confocal sections of a stage III oocyte are shown: (A) animal hemisphere view; (B) vegetal hemisphere view. The images are oriented with the vegetal hemisphere toward the bottom.
(C) Vg1 mRNA was detected in stage III oocytes, by in situ hybridization using a digoxigenin-labeled probe. Shown is a paraffin section, vegetal pole toward the bottom.
(D–F) Stage III oocytes were subjected to in situ hybridization with a fluorescently labeled Vg1 RNA probe, followed by immunocytochemistry with anti-peptide PTB/hnRNP I antibody. Shown is an optical confocal section through the vegetal cortex, viewed in the red channel (D) to detect VgRBP60/hnRNP I and in the green channel (E) to detect Vg1 RNA; the overlap is shown in yellow (F). All scale bars (A–F) represent 50 μm. |
