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Fig. 2. Spatial distribution of Vg1 and SPCs in whole-mount hemisected stage 9 Xenopus blastulae. (A) Vg1, (B) Furin, (C) SPC4, (D) SPC6. Embryos are not oriented specifically. |
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Fig. 3. Spatial distribution of Vg1 and SPCs in sections of stage 9 Xenopus blastulae. Panels show Vg1 expression in red and Furin, SPC4, and SPC6 in green. The double-label images indicate the specific overlapping expression patterns of Vg1with Furin, SPC4, and SPC6 (indicated by white arrows) on the dorsal–vegetal side of the embryo, in the region of the Nieuwkoop center. Adjacent sections were probed with Siamois to confirm dorsal–ventral orientation of the embryos. |