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Display additional annotations [+]
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Fig. 5. Spatial expression pattern of the four representative Gα subunits during early Xenopus tropicalis development. The tissue specific expression patterns of Gα subunit transcripts at different stages of X. tropicalis embryonic development were analyzed by whole-mount in situ hybridization. Probes are indicated to the left of each panel row, and the developmental stages are indicated at the top of each panel column. Animal views are shown for embryos at stage 9; vegetal views for embryos at stage 10, and lateral views for embryos at stages 18–19, 23–24. Vegetal views are shown in the inset of stage 9, and dorsal views are shown in the inset of stage 18–19. White arrows in the stage 10 panels indicate the dorsal marginal zone (dmz). White arrowheads in the stage 23–24 panels indicate the three cranial neural crest routes (ncr) (branchial, hyoidea, and mandibular). Black arrows indicate specific structures labeled as follows: nt, neural tube; p, placodes; and psm, presomitic mesoderm. The embryo images are representative of three independent in situ experiments analyzing 30–50 embryos each time. |
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gna13 (guanine nucleotide binding protein (G protein), alpha 13 ) gene expression in Xenopus tropicalis embryo, assayed via in situ hybridization, NF stage 10.5, blastoporal view, dorsal up. |
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gna13 (guanine nucleotide binding protein (G protein), alpha 13) gene expression in Xenopus tropicalis embryo, assayed via in situ hybridization, NF stage 18, lateral view, anterior left, dorsal up. Inset, dorsal view, anterior up. |
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gna13 (guanine nucleotide binding protein (G protein), alpha 13) gene expression in Xenopus tropicalis embryo, assayed via in situ hybridization, NF stage 23, lateral view, anterior left, dorsal up. |
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Display additional annotations [+]
| Gene |
Clone |
Species |
Stages |
Anatomy |
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gnas
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tropicalis
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NF stage 27
to
NF stage 33 and 34
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otic vesicle
,
somite
,
pharyngeal arch
,
mandibular arch
,
hyoid arch
,
[+]
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gnaq
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tropicalis
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NF stage 27
to
NF stage 33 and 34
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otic vesicle
,
brain
,
hindbrain
,
somite
,
pharyngeal arch
,
[+]
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gnai2
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tropicalis
|
NF stage 27
to
NF stage 33 and 34
|
otic vesicle
,
brain
,
forebrain
,
midbrain
,
hindbrain
,
[+]
|
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Fig. 6. Spatial expression pattern of the four representative Gα subunits during later Xenopus tropicalis development. The tissue specific expression patterns of Gα subunit transcripts at different stages of X. tropicalis embryonic development were analyzed by whole-mount in situ hybridization. Probes are indicated to the left of each panel row, and the developmental stages are indicated at the top of each panel column. Lateral views are shown for embryos at stages 27, and 33–34. Right panels show cross-section in situ hybridization analysis of Gα subunits from stage 27 X. tropicalis embryos. Embryos were coronally sectioned to a thickness of 25–30 μm by a Leica VT1000M vibratome. In left panels, the red dotted lines in the stage 27 embryo indicate the position of the visualized sections. The eye (e) is indicated in white lettering. Black arrows indicate specific structures labeled as follows: b, brain; iv, intersomitic veins; nc, neural crest; nco, notochord; nt, neural tube; ov, otic vesicle; p, placodes; pcv, posterior cardinal vein; rl, rombic lip; and sb, somite boundaries. The embryo images are representative of three independent in situ experiments analyzing 30–50 embryos each time. |
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gna13 (guanine nucleotide binding protein (G protein), alpha 13) gene expression in Xenopus tropicalis embryo, assayed via in situ hybridization, NF stage 27, lateral view, anterior left, dorsal up. |
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gna13 (guanine nucleotide binding protein (G protein), alpha 13) gene expression in Xenopus tropicalis embryo, assayed via in situ hybridization, NF stage 27, transverse section, dorsal up. |
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gna13 (guanine nucleotide binding protein (G protein), alpha 13) gene expression in Xenopus tropicalis embryo, assayed via in situ hybridization, NF stage 33 & 34, lateral view, anterior left, dorsal up. |
