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Figure S1: (a) In situ hybridization confirms the presence of Gα13 and Ric8A transcripts in migrating cranial NC cells at stage 26, as previously reported in Maldonado-Agurto et al. (2011) and Fuentealba et al. (2016). (b) Gα13 gain and loss of function controls. Left panel: Anti-Myc Western blot in embryos injected with a myc-Gα13 mRNA. Right panel: Anti-Gα13 Western blot showing that morphant embryos display diminished levels of endogenous Gα13 protein. GAPDH and α-tubulin were used as loading controls. (c) The rescue assay of the Ric-8A morpholino was performed using a myc-Ric-8A mRNA, as previously reported in Fuentealba et al.(2013). Snail2 was used as a migratory cranial NC marker at stage 23 (left panel). Snail2 expression is unaffected in stage 16 Gα13 morphant embryos (right panel). Legend: Is, injected side; Nis: non-injected side. All experiments shown here were performed in X. tropicalis. |
