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Figure S6: Expression Analysis of G-Protein Beta Subunits. (A) Quantification of the
expression levels of G-protein beta subunits of stage 39 Xenopus tropicalis embryos using
RNAseq. (B) Analysis of Gnb1-MO knockdown efficacy by in vitro transcription/translation. (CG’)
Whole mount in situ hybridization of Xenopus laevis embryos and paraplast sections thereof
demonstrating the expression of G-protein beta subunits in stage 35 embryos. Insets show
close-ups of the pronephric kidney. The expression of Gnb1 in the pronephric kidney was the
most pronounced of all the G-Protein beta subunits and was, thus, selected for the knockdown
studies. |
