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gnb1xenopus animal 

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Experiment details for gnb1

Devic E et al. (1996) Assay

The mRNA encoding a beta subunit of heterotrimeric GTP-binding proteins is localized to the animal pole of Xenopus laevis oocyte and embryos.

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Gene Clone Species Stages Anatomy
gnb1.S x86969 laevis unfertilized egg stage animal
gnb1.S x86969 laevis NF stage 2 (2-cell) animal
gnb1.S x86969 laevis NF stage 8 animal

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  Fig. 5. Expression pattern of XGB1 in Xenopus observed after whole mount in situ hybridization. In situ hybridization was carried out as described in Section 4. (A-C) Cleared oocyte or embryos are positioned with the animal pole on top. They correspond, respectively, to mature oocyte, stage 2 embryo and morula. Note the restriction of the labeling to the animal pole. (D) Vegetal view of a gastrula. The staining is mainly localized in the dorsal region under the blastopore lip (bl), while the vegetal pole is unstained. (E) Dorsal view of a neurula showing specific labeling on the neural plate. (F) Early larval stage; head is on the left, dorsal on the top. An intense staining is observed in neural structures (br, brain; sp, spinal cord) and eye (e). All embryos were depigmented after in situ hybridization.

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Gene Clone Species Stages Anatomy
gnb1.S x86969 laevis unfertilized egg stage to NF stage 8 animal

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  Fig. 6. Transverse sections from whole mount in situ hybridized embryos shown in Fig. 5. (A-C) These transverse sections arc presented with the animal pole on the top, and correspond, respectively, to mature oocyte, stage 2 embryo. and morula. Note that the staining is confined to the animal pole. (D) Section of a gastrula showing a specific labeling above the dorsal blastopore lip (dbl) in the ectoderm. (E) Transverse section of neurula showing the neural plate (np) staining. (F) Transverse section through the trunk of an early larva stage showing the labeling of the neural tube (nt). Abbreviations: gv, germinal vesicle; blc, blastocoele: not, notochord.

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Gene Clone Species Stages Anatomy
gnb1.S x86969 laevis unfertilized egg stage animal

  Fig. 7. Localization of XC/l transcripts during oogenesis between stages III and VI. Staged oocytes are positioned with the animal pole on top. (A-D) Control oocytes. Asymmetry of pigment and vitellus is established at stage IV. (E-H) Whole mount in situ hybridized and cleared oocytes. In situ hybridization was carried out as described in Section 4. Note that the XC/? labeling is homogeneous at stage III and IV, becomes heterogeneous at stage V and is asymmetric at stage VI. (I-L) Transverse sections from whole mount in situ hybridized oocytes. These sections show that the animal localization of XGFI transcript begin to be established at stage V.

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Gene Clone Species Stages Anatomy
gnb1.S x86969 laevis unfertilized egg stage animal

  Fig. 8. Localization of XGB1 transcripts by in situ hybridization on stage VI oocyte sections using s-32P-labeled RNA antisense probe. In situ hybridization was carried out as described in Section 4. Staged oocytes are positioned with the animal pole (AP) on top and the vegetal pole (VP) at the bottom. The position of the germinal vesicle (gv) is shown. Note that the XGfl labeling is exclusively localized in the animal pole.