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Additional File 3. Depletion of XTcf-3 has minor effects on the expression of HMG-box genes. Depletion of XTcf-3 by injection of two picomoles morpholino (Tcf3Mo): 5'-cgctgttgagctgaggcatgatgag-3' (directed against BC077764) into one blastomere of 2-cell stage embryos (the injected side is indicated by an asterisk) has only minor effects on the expression of HMGN1, HMGN2 and HMGX. While HMGN2 and HMGX are laterally expanded (arrow), HMGN1 remains unchanged. Probes for in situ hybridization are as described: HMGN1 and HMGN2 [1], HMGX [2]. |