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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
tcf7l2
|
|
laevis
|
NF stage 14
|
neuroectoderm
,
chordal neural plate
,
neural plate
,
anterior
|
tcf7l2
|
|
laevis
|
NF stage 28
|
forebrain
,
diencephalon
,
forebrain-midbrain boundary
|
cirbp
|
|
laevis
|
NF stage 14
|
neural plate
|
cirbp
|
|
laevis
|
NF stage 28
|
otic vesicle
,
retina
,
hindbrain
,
spinal cord
,
pharyngeal arch
,
[+]
|
cirbp
|
|
laevis
|
NF stage 10.5
|
ectoderm
,
involuted dorsal mesoderm
,
upper blastopore lip
|
hmgb2
|
|
laevis
|
NF stage 14
|
chordal neural plate
,
anterior neural fold
,
optic field
|
hmgb2
|
|
laevis
|
NF stage 28
|
brain
,
hindbrain
,
head region
,
tail bud
,
eye
|
hmgn2
|
|
laevis
|
NF stage 14
|
chordal neural plate
,
eye
,
lateral plate mesoderm
,
optic field
,
posterior placodal area
|
hmgn2
|
|
laevis
|
NF stage 28
|
retina
,
midbrain
,
hindbrain
,
intermediate mesoderm
,
pharyngeal arch
,
[+]
|
tcf7l1
|
|
laevis
|
NF stage 14
|
pre-chordal neural plate
,
chordal neural plate
,
neural plate
,
paraxial mesoderm
|
tcf7l1
|
|
laevis
|
NF stage 28
|
hindbrain
,
tail bud
,
eye
,
visceral pouch
|
tcf7l1
|
|
laevis
|
NF stage 10.5
|
ectoderm
,
mesoderm
,
involuting marginal zone
,
involuted dorsal mesoderm
,
upper blastopore lip
|
|
|
Figure 3. Coexpression of XTcf-3 and putative XTcf-3 target genes. In situ hybridization reveals synexpression of XTcf-3, HMGN1, HMGN2, HMGX and XCIRP throughout early embryonic development. (A) At neurula stages all five genes are co-expressed and demarcate the anterior border of the neural plate (arrowhead). The main expression of HMGX is found in more posterior neural tissue (arrow) adjacent to the XTcf-4 expression field. The coexpression persists during early embryogenesis and is found in tailbud stages in head structures, including brain, eye, otic vesicle and branchial arches. (B) During gastrulation, XTcf-3 and XCIRP are co-expressed at the dorsal blastopore lip (arrowhead upper panel). The higher magnification reveals that both genes are expressed in the involuting mesoderm (anterior most mesoderm is indicated by a red arrowhead). In the endoderm (black arrowhead) underlying the involuting mesoderm, neither XTcf-3 nor XCIRP are expressed. While XTcf-3 is predominantly localized in the mesoderm (black arrow), the highest expression of XCIRP is found in the ectoderm (red arrow). But still substantial amounts of XCIRP RNA are detected in the mesoderm (black arrow). In anterior transversal sections of stage 18 embryos (lower panel) mRNA of both, XTcf-3 and XCIRP, is located in the neuroectoderm (arrowhead). While XTcf-3 is additionally found in the paraxial mesoderm (arrow), the most prominent XCIRP staining apart from the neural plate is located in the epidermis. (C) A higher magnification of transversal sections shows that following XTcf-3 morpholino injection (Tcf3Mo) XCIRP is strongly reduced in the neural plate (arrow) at the injected side (marked with an asterisk). |