| |
Fig. 6. Knockdown of all three PG1 genes affects more posterior Hox genes. Non injected controls (NIC; A,C,E,G,I,K,M,O) and embryos injected on the left-hand side (lhs) with all three Hox PG1 morpholinos (A1/B1/D1MO; B,D,F,H,J,L,N,P) were analysed for Hox gene expression at early tailbud stage. Embryos are shown from the dorsal side, anterior to the top. Hoxb1 expression was lost (A,B: 70%, n=10), and Hoxa2 (C,D: 100%, n=18) and Hoxa3 (E,F: 100%, n=14) expression was almost completely absent. Hoxd3 (G,H: 68%, n=19) and Hoxd4 (I,J: 79%, n=14) neural tube expression was still present, but the pharyngeal arch expression was lost. The more posterior Hox genes (Hoxa5, K,L: 100%, n=10; Hoxc6, M,N: 71%, n=14; Hoxb9, O,P: 93%, n=14) had reduced neural tube expression in the anterior part of the embryo but their posterior expression domains (arrow) and mesodermal expression were unaffected. |
