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Fig. 7. Hoxd1 mRNA rescues Hox expression in PG1 knockdown embryos. Embryos injected on the left-hand side (lhs) with Hoxd1 MO insensitive mRNA (A,D,G,J), all three PG1 morpholinos (B,E,H,K) or a combination of both (C,F,I,L) were analysed for expression of Hoxb2, Hoxa3, Hoxd4 and Hoxc6. Embryos are shown from the dorsal side, anterior to the top. Hoxb2 expression was expanded posteriorly with Hoxd1 mRNA (A: 75%, n=12), lost with the triple PG1 morpholinos (B: 93%, n=28) and rescued when a combination was injected (C: 48% of embryos had downregulated expression, n=33). The other Hox genes were not affected by the Hoxd1 mRNA overexpression. PG1 MO downregulated expression of Hoxa3 (E: 78%, n=9), Hoxd4 (H: 100%, n=9) and Hoxc6 (K: 78%, n=9) and in the rescues this downregulation was reduced to 34%, 20% and 33% for Hoxa3, Hoxd4 and Hoxc6, respectively (F,I,L). |
