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hoxa7xenopus dorsal marginal zone [+] 

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Experiment details for hoxa7

Wacker SA et al. (2004) Assay

Timed interactions between the Hox expressing non-organiser mesoderm and the Spemann organiser generate positional information during vertebrate gastrulation.

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Gene Clone Species Stages Anatomy
hoxa7.L laevis NF stage 12.5 dorsal marginal zone

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  Fig. 2. Hox expression in ventralised and dorsalised embryos. (A) Hoxc-6 in embryos ventralised with UV. WISH (vegetal views of stages 10.5 to 12.5, lateral view of stage 26). Similar results were obtained for Hoxd-1, Hoxb-4, Hoxa-7 and Hoxb-9 (not shown). (B) Diagram showing the onset of Hox expression (detected with WISH) in ventralised (dark blue) and control embryos (light blue). In ventralised embryos, the temporally colinear sequence is still present. (C) Mesodermal Hox expression (WISH) in ventralised embryos. Embryos cut through dorsolateral blastopore lips in controls and the corresponding region in ventralised embryos. Hoxd-1 at stage 11, Hoxc-6 at stage 12, Hoxa-7 at stage 12.5. The white line indicates Brachet’s cleft, separating involuted mesoderm from overlying ectoderm. Neurectodermal Hox staining in control embryos (C, arrowheads) is missing in ventralised embryos (C’). (D) Hox expression is absent in embryos that were dorsalised with LiCl. Dorsalised embryos (LiCl) and controls (con) at stage 12.5 (vegetal views) and stage 26 (lateral views). Results of a WISH for Hoxd-1, Hoxc-6 and Hoxb-9. Analysis of Hoxb-4 and Hoxa-7 showed similar results (not shown). Arrowheads indicate the anterior Hox expression boundary.