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Fig. 5. Sall4 knockdown results in a loss of spinal cord differentiation. (A-H) Whole-mount in situ hybridization of (A,C,E,G) uninjected control embryos and (B,D,F,G) embryos injected with 20 ng Sall4 MO into the right A/D blastomere. (A,B) Expression of otx2, krox20 and hoxb9. Arrows indicate the relative anterior-posterior (A-P) position of krox20 and the anterior limit of hoxb9. Dorsal views with anterior to the top. (C-H) Posterior views of hoxb9 (C,D), hoxc10 (E,F) and hoxd10 (G,H) expression. (I-L) Quantification of A-P patterning defects associated with Sall4 knockdown. (I) Distance between the anteriormost expression of otx2 and the first krox20 stripe in arbitrary units (AU). (J-L) Length of the hoxb9 (J), hoxc10 (K) and hoxd10 (L) expression domains (AU). Error bars indicate s.e.m. Means were compared between left and right sides by Student's t-test (*P<0.05, **P<0.01, ***P<0.001). Data were generated from analyzing all embryos shown in A-H. |
