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Fig. 7. Overexpression of pou5f3 represses neural differentiation. Whole-mount in situ hybridization of (A,C,E,G) uninjected control embryos and (B,D,F,H) embryos injected with 150 pg pou5f3 RNA (50 pg each of pou5f3.2, pou5f3.3 and pou5f3.1 RNAs) into the right A/D blastomere. Red staining is β-galactosidase used as a tracer for RNA injection. (C′-F′) Higher magnification views of the boxed regions in C-F. (A-D) otx2, krox20 and hoxb9 expression in anterior (A,B) or dorsal (C,D) view. (E-H) hoxc10 (E,F) and sox2 (G,H) expression in dorsal view. |
