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Fig. 8. Loss of spinal cord in Sall4 morphants is due to an increase in pou5f3 expression. (A-L) Whole-mount in situ hybridization of (A-D) uninjected control embryos, (E-H) embryos injected with 40 ng Sall4 MO (20 ng/blastomere at the 2-cell stage), or (I-L) embryos injected with 40 ng Sall4 MO, 20 ng Pou5f3.2 MO, 10 ng Pou5f3.3a MO, 10 ng Pou5f3.3b MO, and 20 ng Pou5f3.1 MO. (N) Embryos injected with 20 ng Pou5f3.2 MO, 10 ng Pou5f3.3a MO, 10 ng Pou5f3.3b MO and 20 ng Pou5f3.1 MO. Expression is shown for (A,E,I) otx2, krox20 and hoxb9, (B,F,J) hoxc10, (C,G,K) hoxd10 and (D,H,L,N) sox2. Dorsal views with anterior to the top. (M) Quantification of posterior neural gene expression as measured by expression domain length in arbitrary units (AU). White, uninjected control embryos. Gray, embryos injected with 40 ng Sall4 MO (20 ng/blastomere at the 2-cell stage). Black, embryos injected with 40 ng Sall4 MO, 20 ng Pou5f3.2 MO, 10 ng Pou5f3.3a MO, 10 ng Pou5f3.3b MO and 20 ng Pou5f3.1 MO. Error bars indicate s.e.m. Means compared with uninjected control by one-way ANOVA followed by Tukey post-hoc analyses (***P<0.001). Data were generated from analyzing all embryos shown in A-C, E-G, and I-K. |
