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hoxd3xenopus rhombomere R5 

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Experiment details for hoxd3

Retinoid signalling is required for information transfer from mesoderm to neuroectoderm during gastrulation.

Retinoid signalling is required for information transfer from mesoderm to neuroectoderm during gastrulation.

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Gene Clone Species Stages Anatomy
hoxd3.L laevis NF stage 20 rhombomere R5

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  Fig. 2. Retinoid depletion causes radical molecular truncation of the posterior hindbrain by the end of gastrulation. (Top panel) Whole- mount in situ hybridizations (wISH) on st. 20 Xenopus laevis embryos (A- F). The upper row shows non-treated embryos (indicated by control) and the bottom embryo is treated with 10-6 M AGN (indicated by AGN). All views are dorsal and anterior at the top. (A) Hoxb-1, arrowhead indicates hindbrain expression; (B) Hoxd-3, ar- rowhead indicates hindbrain expression; (C) En2, Krox-20 and Hoxb-4, top arrowhead indicates En stripe, bottom arrowheads indicate Krox-20 stripes and bar indicates Hoxb-4 stripe; (D) Hoxa-5, arrowhead indicates hindbrain expression and bar indicates spinal cord expression; (E) Krox-20 and Hoxc- 6, arrowhead indicates posterior Krox-20 stripe, bar indicates Hoxc-6 expression; (F) Otx-2 and Xcad3, bar indicates gap between Otx-2 (anterior) and Xcad3 (posterior) expression patterns; (G) XlPOU2, arrowhead indicates hindbrain expression and bar indicates spinal cord expression. (Bottom panel) Whole-mount in situ hybridizations on st. 13 Xenopus laevis embryos (H-M). The upper row shows non-treated embryos (indicated by control) and the right embryo is treated with 10-6 M AGN (indicated by AGN). All views are dorsal and anterior at the top. (H) Hoxd-1; (I) Hoxa-1; (J) Hoxb-1; (K) Hoxd-3; (L) Krox-20 (anterior stripes) and Hoxb-4; (M) Krox-20 (anterior stripes) and Hoxc-6. Arrows in pictures (L,M) localise sparse cells representing the posterior stripe of Krox-20.