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Figure 2. Over-expression of kazrinA has no detectable effect on the development of Xenopus tropicalis. A: Levels of kazrinA protein are elevated in stage-28 embryos previously injected with 900 pg kazrinA mRNA but not stage-39 embryos. Extracts of single uninjected (U) or injected (I1 and I2) embryos were analysed by Western blotting for expression of kazrinA. Arrow, position of full-length kazrinA. B,C: Immunocytochemical analysis confirms that levels of kazrinA protein are elevated in stage-28 embryos. B is a control embryo and C an embryo injected with 900 pg kazrinA mRNA. D,E: Sections of a control embryo (D) and of an embryo previously injected with 900 pg kazrinA mRNA (E). Both develop normally. F,G: Higher-power views of the embryos in D and E, respectively. Scale bars = 75 mm (B,C), 100 mm (D,E), 50 mm (F,G). |
