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Figure 4: Localization of the asymmetric gene Xnr-1 (nodal) is not affected by HCN4-
DN and ZD7288.
(A) Representative images of approximately stage 21 embryos assayed for Xnr-1
(nodal) expression by in situ hybridization and quantification of area of nodal
expression. Red dotted line is midline and L representing left-side, R representing
right-side, H representing head and T representing tail of embryos. Scale bar = 0.25
mm. (i) is no probe (negative) untreated control, (ii) Control embryos with Xnr-1 signal
– red arrow, (iii) ZD7288 treated (from stage 1-10) embryos with Xnr-1 signal – red
arrow, (iv) HCN4-DN mRNA injected (in both blastomeres at 2-cell stage) embryos
with Xnr-1 signal – red arrows, and (v) Quantification of area of Nodal expression in
embryos showed no significant change in the area of Nodal expression in HCN4-DN
mRNA injected and ZD7288 treated embryos. N>10, Data was analyzed by one-way
ANOVA, n.s. – Non-significant. (B) Representative images of approximately stage 23
embryos assayed for Lefty expression by in situ hybridization and quantification of
area of lefty expression. Red dotted line is midline and L representing left-side, R
representing right-side, H representing head and T representing tail of embryos. Scale
bar = 0.25 mm. (i) is no probe (negative) untreated control, (ii) Control embryos with
Lefty signal – yellow arrow, (iii) ZD7288 treated (from stage 1-10) embryos with Lefty
signal – yellow arrow, (iv) HCN4-DN mRNA injected (in both blastomeres at 2-cell
stage) embryos with Lefty signal – yellow arrows, and (v) Quantification of area of Lefty
expression in embryos showed no significant change in the area of Lefty expression
in HCN4-DN mRNA injected and ZD7288 treated embryos. N=10, Data was analyzed
by one-way ANOVA, n.s. – Non-significant. |
