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Figure S1. Myo1d is required for LR axis formation, Related to Figure 1.
(A-H) nodal1 (A-D) and pitx2 (E-H) expression in wildtype (A, E) and myo1d morphant (B-D, F-H) embryos, as
determined by WM-ISH with antisense probes for nodal1 (A-D) and pitx2 (E-H).
(I, J) Downregulation of Myo1d protein in myo1d morphants. (I) Tadpole lysates were probed with Mab4E12 before
(left) and after (right) affinity purification (ap). (J) Embryos were injected at the 2-4 cell stage with 1 ng of AUG-MO or
an antisense MO containing 5 mismatches (MM-MO). Affinity purified Mab4E12 was used to probe western blots
containing lysates from stage 28 embryos.
(K, L) Lefty1 mRNA expression in wildtype (K) and myo1d morphant (L) specimen, as shown by transversal
histological sections of WM-ISH stained embryos at stage 24. fp, floor plate; hc, hypochord; MM-MO, mismatch MO. |
