| |
Figure S3: Early markers of dorso-ventral development and cilia cell fate.
(a) In situ hybridization for early developmental markers at stage 10-11 Xenopus
embryos in uninjected controls (top row) and wdr5 morphants (bottom row). All vegetal
views with dorsal to the left (at stage 10-11).
(b) In situ hybridization for sox2 at stage 14, with an anterior view with dorsal to the top.
(c) In situ hybridization for dand5 and xnr1 in the Xenopus LRO at stage 16. Margin of
the LRO is indicated by dashed white lines.
(d) In situ hybridization for shh and lefty at stage 16, with an anterior view with dorsal to
the top.
(e) In situ hybridization for rfx2 and dnah9 in the Xenopus LRO at stage 16. Margin of
the LRO is indicated by dashed white lines.
(f) Quantification of in situ hybridization for the midline marker dand5, xnr1, shh, lefty,
rfx2 and dnah9. Abnormal is either reduced or absent expression compared to wildtype
expression (normal).
Experiments were repeated 2-3 times. ‘n’ = number of embryos. |
