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FIGURE 6.
Expression patterns of mesoderm and organizer marker genes in Dhrs3 morphants and embryos treated with 1 μM atRA during gastrulation. Embryos were injected at the two-cell stage with either 30 ng of Dhrs3 MO or 30 ng of Dhrs3 MO plus 1 ng of dhrs3 mRNA or treated with 1 μM of atRA at gastrulation stages. Whole mount in situ hybridization was used to visualize mesoderm marker gene brachyury (bra) (A1�A4), organizer marker genes lhx1 (B1�B4), and goosecoid (gsc) (C1�C4). Expression of bra was disrupted in Dhrs3 morphants (A2, arrow) and in embryos treated with atRA (A4, arrow). Injection of 1 ng of dhrs3 mRNA rescued the disruption (A3). The expression intensity of lhx1 was elevated by Dhrs3 knockdown (B2) and atRA treatment (B4). Compared with the control embryos (C1), the expression domain of gsc was closer to the blastopore in Dhrs3 morphants (C2) and was abolished by atRA treatment (C4). Dotted line outlined the blastopore. |
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Figure S2: dhrs3 suppresses the formation of pronephros. (A) The pronephros marker gene lhx1 indicated the position of the pronephros in stage 35 embryos. lhx1 was also expressed in the neural tube. (B) The expression of lhx1 in the pronephros region was enhanced by dhrs3 knockdown, suggesting that dhrs3 exerted an inhibitory role on pronephros development. (C) Overexpression of dhrs3 inhibited the development of pronephros, as showed by lhx1 staining in that region.
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