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Fig. 3. Spatial distribution of msx1 transcripts during early Xenopus development. Whole-mount in situ hybridization of albino embryos using a digoxigenin-labeled antisense msx1 probe. msx1 transcripts are detectable from midgastrula stages (stage 11; A,B). Dorsal (A) and lateral (B) views indicate the localization of msx1 transcripts in the ventral animal and marginal regions of the embryo. Dorsal (C) and lateral (D) views at early neurula stages indicate that the expression of msx1 gradually divides into ventral and dorsal regions, and that the dorsal expression is restricted to the border of epidermis and neural plate. a, anterior; p, posterior; d, dorsal; v, ventral. (E,F) Dorsal and ventral views of stage 17 embryos, respectively, and anterior is up. As the neural tube closes, the expression of msx1 is observed in the dorsal neural tube (E) and the heart and around blastopore on the ventral side (F). At tailbud (G) and tadpole (H) stages, expression is detected in fin, cement gland, dorsal neural tube, otic vesicle and, broadly, in the ventral region. |
