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Fig. S5. Depletion of Tbx2 increases the rate of cell proliferation in the pronephric nephron. (A) Phospho-histone H3 staining of the pronephric field in a Tbx2-depleted embryo. Membrane-targeted RFP was co-injected as a lineage tracer. (B) In situ hybridization showing the expression of NMyc1 in the Tbx2 MO (10 ng)-injected and uninjected control sides of an embryo. A transverse section of the embryo shown in the upper panel at the level of the dashed line is shown beneath. The dotted line encloses the pronephric duct (pd). (C) Hematoxylin and Eosin staining of the pronephric field in the Tbx2-depleted embryo. |
