||Fig. 2. Nutrients are necessary for acquisition of the committed neural progenitor fate but not for the maintenance of the most immature
stem/progenitor cells. (A) Proneural gene expression levels decreased following ND, as did levels of the progenitor markers sox2 and n-Myc (B). Dashed line in B indicates the central domain of the CMZ. n=8, 32 embryos/condition. (C) Expression of the eye field transcription factor gene rx1 remained unchanged. n=4, 16 embryos/condition. (C) c-Myc gene expression levels remained unchanged; however, the domain of c-Myc gene expression was reduced in the central CMZ of ND embryos. n=8, 32 embryos/condition. (D) Expression levels of the stem cell marker genes hes1 and hes2 remained unchanged in ND conditions. n=4, 16 embryos/condition. Scale bars: 50 μm (A,B,D); 50 μm (C; rx1) 25 μm (C; c-Myc).