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myf6xenopus   

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Experiment details for myf6

Myogenic waves and myogenic programs during Xenopus embryonic myogenesis.

Myogenic waves and myogenic programs during Xenopus embryonic myogenesis.

Gene Clone Species Stages Anatomy
myf6.L laevis NF stage 16 to NF stage 18 paraxial mesoderm

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  Figure 2. A lateral myogenic population differentiates at the trunk level during neurulation. A: Whole-mount in situ hybridization of myogenic regulatory factor (MRF) and muscle-marker genes during the second part of neurulation. Brackets indicate the lateral myogenic population. Dorsal views. Vertical lines define the limit between anterior and trunk region. The anterior side of the embryos is on the left. B: Transverse sections at the trunk level of embryos submitted to in situ hybridization for Myod, Des (stage 16 and 17/18) or Myogenin mRNA detection (stage 17/18). 12/101 antibody detects differentiated muscle cells at stage 16 and 17/18 (blue). C: Localization of Myod mRNA on dorsal view and on transverse section at stage 21/22. Arrows and arrowheads indicate the dorsomedian and ventrolateral Myod expression domains, respectively. Dotted lines define the median differentiated muscle fibers. Nc, notochord. Download figure to PowerPoint.

Gene Clone Species Stages Anatomy
myf6.L laevis NF stage 22 to NF stage 23 presomitic mesoderm , somite
myf6.L laevis NF stage 26 to NF stage 27 somite

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  Figure 3. Expression of myogenic regulatory factor (MRF) at tailbud stages. Expression of the four MRFs at stages 22–23 in comparison with Des (A) and at stages 26-27 (B) determined by whole-mount in situ hybridization with labeled antisense probes against Myf5, Myod, Mrf4, Myogenin, and Des mRNAs. The anterior side of the embryos is on the left. Lateral views. Arrowheads in A: Myf5 mRNA staining at the dorsomedian and ventrolateral somitic borders. The probe is indicated in each panel. Time for Myogenin mRNA revelation was longer than for the other MRF at these stages.Download figure to PowerPoint

Gene Clone Species Stages Anatomy
myf6.L laevis NF stage 29 and 30 to NF stage 46 somite

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  Figure 5. The full myogenic program is expressed during the second myogenic wave. A: Comparison of each myogenic regulatory factor (MRF) mRNA expression from stage 30 to 40. Myf5 and Myogenin are easily detectable in the dorsal and ventral borders of the dermomyotome at stage 30 and 34, respectively. The four MRFs are successively expressed during hypaxial myogenesis (arrows), which gives rise to the ventrolateral musculature. B: The four MRFs are expressed at the epaxial region of the somites (arrowheads) during the second myogenic wave. C: Myf5 and Myod genes are expressed in the presomitic mesoderm of the caudal region. Myogenin expression is restricted to the dorsomedian and ventrolateral regions of the somites and Mrf4 accumulates in the differentiated muscle cells. D: Myf5 staining appears as dots at stage 40, probably reflecting the formation of a third wave of myoblasts. Myf5 is expressed in the anterior and posterior region of the somites (arrows) at stage 45, whereas Mrf4 mRNA staining is concentrated in the central region of the elongated cells which span entirely the somite. Lateral views, the anterior side of the embryos is on the left. The probe is indicated in each panel.Download figure to PowerPoint

Gene Clone Species Stages Anatomy
myf6.L laevis NF stage 35 and 36 somite , musculature of face , m. quadrato-hyoangularis primordium , m. interhyoideus primordium
myf6.L laevis NF stage 40 branchial arch , musculature of face , m. levator mandibulae articularis primordium , m. intermandibularis primordium , m. quadrato-hyoangularis primordium , [+]

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  Figure 8. Distinct myogenic programs are expressed during craniofacial myogenesis. A: Schematic representation of head muscle anlagen according to Ziermann and Olsson (2007). B: Expression of Myf5, Myod, Myogenin, and Mrf4 mRNAs in head muscles anlagen. Myf5 is expressed neither in the M. levatores mandibulae, nor in the M. quadrato-hyoangularis and orbitohyoideus anlagen (black arrows). Myod transcripts are not detected in the dorsal part of branchial muscle anlagen and in the intermandibularis anlage (brackets). Green arrows design extraocular muscle anlagen. C: Expression of Des, Actc, and Ckm, as well as MyhE3 and MyhE19 coding, respectively, for embryonic fast and slow myosin heavy chain (Radice and Malacinski, 1989). St, stage. For each probe, ventral view in the left panel, lateral view in the right panel with the anterior side of the embryos on the left. h, heart. Download figure to PowerPoint.