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Figure 3. Unilateral microinjection of XMRF4 into one cell at the two-cell stage. A: Control EF1-α RNA-injected embryo (left) and XMRF4 RNA-injected embryo (right). Embryos are analyzed at stage 23. Arrows indicate a dysmorphogenesis of neural tube (left) and a lateral bulge on the side injected (right). B,C: Cross-sections of the same embryos: control EF1-α RNA-injected embryo (B), XMRF4 RNA-injected embryo (C) reacted with S35- MHC E3 (larval myosin heavy chain) cRNA. Note the enlargement of myotome at the level of the lateral bulge (arrow) in the XMRF4 RNA-injected embryo. NT, neural tube; NC, notochord; S, somite. |
