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Fig. 1. Knockdown of p38α prevents heart development. (A) Anterior transverse sections of stage 38 control embryos with ventral heart structures, embryos injected with p38α AMO missing heart structures and embryos injected with p38α AMO and a transcript encoding mouse p38α with some heart structures. Sections were hematoxylin–eosin stained. (B) Anterior transverse sections of stage 46 control embryos or of embryos injected with p38α AMO. Sections were immunostained with antibodies to Troponin I (red) and counterstained with TO-PRO-3 (nuclear, blue). (C) Left panel: In situ hybridization staining of stage 30 embryos with a probe detecting myosin heavy chain α. At the two-cell stage, one blastomer was injected with p38α AMO (n = 38). Control group (n = 34) was not injected. Right panel: Embryos were injected with p38α AMO, DMZ explants (n = 18) were isolated and grown to stage 30. Total RNA was isolated and RT-PCR analysis was performed. |
