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Fig. 5. Rdd3/4 MO does not suppress the expression of angiopoietic differentiation markers. Two right side blastomeres of 4-cell-stage embryos were injected with 4.5 pmol rdd3/4 MO and the expression of flk-1 (A and B), tie-2 (C and D), lim-1 (E and F), hex (G), myosin heavy chain-α (MHC-α) (H), scl (I) and α-globin (J) was examined in the resultant embryos at the tailbud stage (st. 32). Levels of expression of each gene in the right and left sides of embryos were compared. Note that expression of α-globin was suppressed in the injected side of the embryos (arrowhead in J), but the expression of flk-1, tie-2, scl, hex, MHC-α and lim-1 was not affected by injection (arrowheads in B, D and F). In another series of experiment, embryos were injected with β-gal RNA (0.5 ng/embryo) alone (K and M) or β-gal RNA with rdd3/4 MO (4.5 pmol) (L and N) in the right side of embryo and were allowed to developed until st. 32. Fixed embryos were stained with Red-Gal (red dye as a tracer), followed by in situ hybridization to detect α-globin expression. M and N show the dissected embryos of K and L, respectively. |
