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Fig. 7. DM-GRASP and Wnt11-R are functionally linked. (A) Wnt11-R MO was unilaterally injected at the 8-cell stage and expression of cardiac marker genes was monitored at stage 28. Wnt11-R does not affect expression of the early cardiac marker genes Nkx2.5, Isl-1, and Tbx20, but markers of differentiated cardiomyocytes of the FHF such as TnIc, cActin, and MHCα. Bar graphs depict the quantitative presentation of the observed phenotype. Error bars are standard errors of the means. N = number of examined embryos, n = number of independent experiments. (B) Co-injection of RNA coding for DM-GRASP partially reverted the phenotype triggered by the Wnt11-R MO. Bar graphs depict the quantitative presentation of observed phenotype. Error bars are standard errors of the means. N = number of examined embryos, n = number of independent experiments. |
