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Fig. 4. Htt, HAP1, and MLK2 activate ND. (A) Xenopus embryos were injected in one blastomere at the two-cell stage with various combinations of mRNA as indicated. Formation of ectopic neurons induced by ND was visualized as dark staining at the tail-bud stage by whole-mount immunostaining with an antibody against the neuronal marker N-CAM. A limiting amount of ND mRNA was used to elicit a minimal neurogenic response when injected alone (indicated by the arrow, Left Center). Results shown are representative of at least three independent experiments. (B) Quantitation of ectopic neurogenesis induced by ND. Bar graph shows mean percent of Xenopus embryos with ectopic neurons ± SEM of at least three independent experiments performed as described above. Dark areas show mean percent of Xenopus embryos with extensive and intense ectopic neurogenesis. |
