| |
Fig. 2. Whole-mount immunocytochemistry of N-CAM expression
in stage-30 embryos. The embryos were labelled by antibodies
directed against Xenopus N-CAM. (A) Control embryo. N-CAM is
detected in the eyes and the entire central nervous system.
(B) Noggin-injected embryo. Ectopic neural tubes are revealed by
anti-N-CAM antibodies. (C) Embryo injected with RNAs encoding
noggin and XFD. Small amounts of N-CAM-positive cells are
present. (D) Embryos injected with XFD RNA in the dorso-animal
blastomeres at the 8-cell stage. Small amounts of neural tissues and
fused eyes (arrowheads) are present. (E) Embryos injected with XFD
RNA in the dorsovegetal blastomeres at the 8-cell stage. Notice the
trunk deficiency and unaffected head region with normal eyes
(arrowheads). Scale bar, 500 mm. |
