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Fig. 3. Phenotypes of XASH/XE12-injected embryos. (A). Direct dorsal view of a whole-mount embryo injected with 90 pg ofbXASH-3 mixed
with XE12 in the right side (anterior to right), and stained for the expression of NCAM mRNA by in situ hybridization (Harland, 1991). The
neural tissue on the injected side (brackets) is obviously much wider along its entire length including hindbrain (hb) and spinal cord (sc) than
that on the uninjected side. (B) Direct dorsal view of a late neural plate embryo shows a striking reduction in ectodermal Xtwist expression (in
white boxes) on the XASH-3/XE12-injected right side (brackets). Anterior (a) is to the right. (C) A section through the spinal region of an
embryo like that in A. In this section, also labeled for NCAM expression, we note the lateral expansion (between arrowheads) of the neural
tissue on the injected right side (brackets). The normally sized notochord (nc), marking the midline, is visible directly below the neural tube
(nt). (D) Expansion of the neural tube (nt) in a XASH-1/XE12-injected embryo. Here the tube was photographed using Nomarski optics. The
white vertical bar marks the midline of the neural tube. |
