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Fig. 4. Microinjection of dullard Mo suppressed expression of neural markers. Control morpholino oligonucleotide (A, C, E, G, I, K) or dullard Mo
(B, D, F, H, J, L) (40 ng) together with lacZ mRNA (200 pg) as a tracer was injected into a dorsal animal blastomere of8-cell embryos. Embryos were
harvested at the early tail-bud stage (A–D) or the neurula stage (remaining panels) and analyzed by whole mount in situ hybridization with the
indicated probes. In the dullard Mo-injected side ofthe embryo, expression ofthese markers is downregulated (B, D, F, H, J, L), while no change is
observed in the control morpholino oligonucleotide-injected embryos (A, C, E, G, I, K). (A and B) lateral view, (C–F) dorsal view, (G–L) anterior
view. |
