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FIG 1. Indirect immunofluorescent images on a video monitor showing NCAM in a coronal section of neural plate stage 1405 XWL’
embr: yo (a) and a sagittal section of a neural plate stage 16117 embryo (b). Strong immunofluorescence was seen on the neural ectoderm
adjacent mesoderm but was absent from endoderm and nonneural ectoderm. Magnification bar is 100 pm. |
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FIG. 2. Immunocytochemical detection of NCAM at the initial neural tube stage on a tissue section of a stage 20 Xenopus embryo. A coronal
section through the closed neural tube (NT) showing NCAM immunoreactivity over the whole neural tube with strong staining of the radial
profiles of the cells of the neuroepithelium. The notochord (N) and somites (S) are not immunoreactive. Magnification bar is 100 pm. |
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FIG. 6. Sagittal section through a Xenopus embryo at stage 26 showing NCAM immunochemical staining of the brain (N) and mandibular
branch of the trigeminal nerve (arrow). The pigment in the sucker (S) and skin is melanin. Magnification bar is 100 pm. |
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FIG. 3. Horizontal section through a somite of a Xenopus embryo at stage 32, during neural innervation of the somitic muscle fibers. Strong
NCAM immunoreactivity is observed at sites of neuromuscular contacts (arrows). Magnification bar is 100 pm. |
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FIG. 4. Coronal section of the rhomhencephalon of a stage 32 Xenopus embryo showing NCAM immunoreactivity on nerve cells (N) and
neuroepithelial germinal cells (G) and roots of cranial nerves VII/VIII (star) and more intensely on the longitudinal fiber tracts (arrows). The
pigment in the surface ectoderm is melanin. Magnification bar is 100 gm. |
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FIG. 5. Coronal section of spinal cord of Xenopus at stage 32 showing strong immunochemical staining on profiles of nerve cells (N) on the
longitudinal fiber tracts (L), commissural fibers (C) and spinal nerve roots (arrows). Pigment in the skin is melanin. Magnification bar is 100um |
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FIG. 7. Coronal section through the rhombencephalon of a stage 39 Xenows embryo showing NCAM immunoreactivity on profiles of cells in
the brain (N) and ganglia of cranial nerve V (arrows). Pigment in the skin is melanin. Magnification bar is 100 Frn. |