| |
FIG. 2. Localization of N-CAM mRNAs by whole mount in situ
hybridization. (A) Uninjected Xenopus embryos. (B) Embryos injected
with 10 pg Xnr-1 mRNA. N-CAM mRNAs were expressed in
both primary axis (arrow) and secondary axis (arrowhead). Other
partial secondary axes (induced by noggin, follistatin, both Xnr-1 and
noggin, both Xnr-1 and follistatin) were similar (data not shown). (C)
Embryos injected with 10 pg Xnr-1 mRNA and 10 pg noggin mRNA.
N-CAM mRNAs were expressed in both primary axis (arrow) and
secondary axis (arrowhead). (D) Dorsal-expanded embryos formed by
injection with 10 pg Xnr-1 and 100 pg follistatin mRNA. N-CAM
mRNAs expressing regions were seen to spread (asterisk). |
