Gene Expression Search Experiment

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Gene/Clone	Species	Stage	Anatomy Item	Experimenter
neurod4	xenopus		posterior placodal area [+] amphibian papilla,anterior crista,auditory hair cell,auditory ossicle,auditory tube,basilar papilla,endolymphatic duct,footplate of the stapes,inner ear,lagena,lateral crista,middle ear,osseous labyrinth,otic capsule,oval window,pars externa of the stapes,pars interna of the stapes,pars media of the stapes,posterior crista,prootic bone,saccular macula,semicircular canal,stapes,tympanic annulus,tympanic disk,vestibular apparatus,vestibule,anterior lateral line ganglion,anterior lateral line placode,auditory apparatus,hyomandibular lateral line,infraorbital lateral line,infraorbital lateral line primordium,lateral line placode,occipital lateral line,otic capsule,otic placode,otic vesicle,supraorbital lateral line,trunk lateral line,vestibulocochlear ganglion

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Experiment details for neurod4

Xath5 regulates neurogenesis in the Xenopus olfactory placode.

Xath5 regulates neurogenesis in the Xenopus olfactory placode.

Gene	Clone	Species	Stages	Anatomy
neurod4.L		laevis	NF stage 17	posterior placodal area
neurod4.L		laevis	NF stage 20	posterior placodal area

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Fig. 1. sequential activation of basic helix-loop-helix (bHLH) and repeat HLH (rHLH) transcription factors during early olfactory development. A: X- NgnR-1 was expressed at stage (St) 15 in the region of the lateral anterior neural ridge, which is fated to produce the olfactory placodes. B: This expression was maintained as the placode develops, shown at stage 20. C: The rHLH gene Xebf2 was first detected at stage 16 within this same region. D: Xebf2 expression also persisted within the olfactory placode in the same domain as that of X-NgnR-1, shown at stage 20. The two Xenopus atonal homologues Xath3 and Xath5 were expressed beginning at stage 17, with Xath5 staining readily apparent at stage 18 (E and G, respectively). F,H: Their expression persisted within the presumptive olfactory region and was still present at stage 20. I: Xebf3 was not expressed in the nascent olfactory region until stage 20, concomitant with the earliest N-tubulin expression within the developing olfactory placodes (data not shown). All embryos are shown in an anterior view, dorsal toward the top; arrows indicate olfactory expression.