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Fig. 3. Expression of Xath2 during Xenopus development. (Top panels) RNAase protection analysis of Xath2 transcripts during embryogenesis and in adult organs. The FGF receptor is used as an internal control. (Bottom panels) Whole-mount in situ hybridization using Xath2 antisense RNA on stage 35 embryos. (A) Lateral view; (B) dorsal view; (C) parasagittal section; (D) transverse section; (E) horizontal section; (F) double labeling for BrdU incorporation (green) and Xath2 expression (black) on a horizontal section. E, egg. |
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Fig. 4. Expression of Xath2 compared with other genes expressed in the dorsal forebrain. (Top panels) Stained double in situ hybridized embryos (st. 35) with Xath2 (black) and Eomesodermin (red) were gelatin-embedded and vibratome-sectioned at 30 μm. (A) Horizontal and (B) parasagittal sections. (Bottom panels) Double in situ hybridization on sections with Xath2 (red) and the bHLH genes Xath3 and NeuroD (black). (C,D) Horizontal sections of st. 35 embryos. (E,F) Transverse sections of dissected brains (st. 54) at the level of the telencephalon. |