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Fig. 5.
Nosip deficiency results in neurogenesis and brain differentiation defects in Xenopus (A) pax6-, neurogenin- and tubb2b-specific WMISH of Xenopus embryos at stage 13 after unilateral injection of 23 ng Nosip MO or Control MO as indicated, injected side at the right, arrowhead points to the posterior neural plate (B) Statistical evaluation of the data shown in A (C)tubb2b-specific WMISH of Xenopus embryos at stage 17 treated as described in A, arrowhead points to reduced number and misaligned tubb2b-positive cells (D) Statistical evaluation of the data shown in C (E)emx1-, en2-, and egr2-specific WMISH of Xenopus embryos at stage 23 treated as described in A, arrowheads point to reduced brain marker gene expression (F) Statistical evaluation of the data shown in E (G)Xenopus embryos at stage 23 stained with a pH3-specific antibody after unilateral injection of Nosip MO or Control MO as indicated (H) Statistical evaluation of the data shown in G (I) TUNEL staining of Xenopus embryos of stage 23 after unilateral injection of Nosip MO or Control MO as indicated (J) Statistical evaluation of the data shown in I.
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