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nkx2-1xenopus   

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Experiment details for nkx2-1

Retinoic acid-induced expression of Hnf1b and Fzd4 is required for pancreas development in Xenopus laevis.

Retinoic acid-induced expression of Hnf1b and Fzd4 is required for pancreas development in Xenopus laevis.

Gene Clone Species Stages Anatomy
nkx2-1.L laevis NF stage 35 and 36 to NF stage 37 and 38 forebrain , thyroid primordium , lung bud

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  Fig. S11. Effects of Hnf1β down- or upregulation on the expression of various endodermal organ marker genes Four-cell stage embryos were injected with RNA encoding β-galactosidase and either (A) Hnf1β-morpholino or control-morpholino, or (B) RNA coding for Hnf1β-GR, vegetally into the two dorsal blastomeres. At stage 36/37 embryos were used for WMISH against Pdx1 (pancreas, duodenum), hHex (liver), Nkx2.1 (thyroid, lung) and (B) Darmin (posterior endoderm).

Gene Clone Species Stages Anatomy
nkx2-1.L laevis NF stage 37 and 38 to NF stage 39 thyroid primordium , lung bud

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  Fig. S12 Effects of Fzd4/Fzd4s downregulation on the expression of various endodermal organ marker genes Eight-cell stage embryos were injected with RNA coding for β-galactosidase and either Fzd4/ Fzd4s-morpholino or the corresponding mismatch-morpholino. At stage 38/39, embryos were used for WMISH against Pdx1 (pancreas, duodenum), hHex (liver), Nkx2.1 (thyroid, lung) and IFABP (stomach, intestine).