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Fig. 6. Influence of Zyxin on Shh/Gli1-responsive genes in embryos with overexpressed Shh. 8-cells embryos were injected into the right dorsal blastomere with the following amount of mRNAs indicated in the top line: Zyxin�150 pg/embryo; Shh�15 pg/embryo; Zyxin-δ2LIM�150 pg/embryo, along with the living tracer, FLD. After in situ hybridization of embryos at stage 25 with probes to Pax6 (A�D), Dbx1 (F�I), Nkx2.2 (K�N) and Foxa2 (P�S), sagittal projections of expression domains of each gene were measured on the left (control) and right (injected) sides of the neural tube and percentage change of expression on the injected side (δ) was calculated (see diagrams in E and O). Mean value and standard deviation of the expression domain changes for all embryos injected by the same set of mRNAs are shown on E, J, O and T by columnar diagrams. Red bars on photos indicate borders of the expression domains on the injected and non-injected sides of embryos. Dotted line indicate border of the neural tube. Red dotted lines on schematic representation of neural tube section on E indicates normal positions of the ventral borders of the expression domains of Pax6, n�number of embryos analyzed in each case. |
