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Fig. 6. Neural patterning modulated by DHCR7. (A) Schema of unilateral injection experiment. Eight-cell stage Xenopus embryos were unilaterally (right side) microinjected with 20 ng of control MO (B,E,H) or 20ng of DHCR7 MOs alone (C,F,I) or with 500 pg of DHCR7 rescue mRNA (D,G,J). Transverse section of whole-mount in situ hybridization of the tailbud stage embryos microinjected with DHCR7-MOs shows ventralization of neural tube as marked by Shh (B-D), Nkx2.2 (E-G), FoxA2 and Dbx1 (H-J) expression. Arrowheads indicate the dorsal expression limit of the indicated markers. (K) Summary of ventralization of neural tube by loss of DHCR7. |
