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nkx2-2xenopus dorsal pancreatic bud [+] 

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Experiment details for nkx2-2

Jarikji Z et al. (2009) Assay

The tetraspanin Tm4sf3 is localized to the ventral pancreas and regulates fusion of the dorsal and ventral pancreatic buds.

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Gene Clone Species Stages Anatomy
nkx2-2.S laevis NF stage 37 and 38 dorsal pancreatic bud

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  Fig. 3. Microarray analysis of dorsal and ventral pancreatic buds. (A) Schematic illustrating the experimental plan. Individual dorsal and ventral pancreatic buds were isolated from NF38 tadpoles. (B) Diagram of isolated liver and pancreas tissue samples, illustrating the distinct dorsal and ventral regions of the early pancreas, shortly after fusion. Dark blue, dorsal pancreas; light blue, ventral pancreas; pink, liver. (C-F) Whole-mount in situ hybridization on isolated liver/pancreas tissue at NF40 of selected pancreatic genes. dp, dorsal pancreas. (G) RT-PCR analysis of selected pancreatic genes in dorsal and ventral pancreatic bud samples used for the microarray.