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nodal1xenopus   

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Experiment details for nodal1

More than 95% reversal of left-right axis induced by right-sided hypodermic microinjection of activin into Xenopus neurula e...

More than 95% reversal of left-right axis induced by right-sided hypodermic microinjection of activin into Xenopus neurula embryos.

Gene Clone Species Stages Anatomy
nodal1.L laevis NF stage 24 lateral plate mesoderm

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  FIG. 5. Left-handed expression of Xnr-1 and Xenopus Pitx2 are altered by the hypodermic injection of activin into the right flank. (A) An untreated embryo. Normal expression of Xnr-1 in the left LPM is observed (lateral view). (B) A case of bilateral expression of Xnr-1 in both LPMs induced by right injection of 10 pg activin (dorsal view). (C) Left expression of Pitx2 after left injection of 10 pg activin at stage 15–16. (D) Bilateral expression of Pitx2 after right injection of 10 pg of activin at stage 15–16. (E) A cross section of a tailbud-stage embryo showing bilateral expression of Xnr-1 after the right injection. (F) A section of a tailbud-stage embryo showing bilateral expression of Pitx2 after the right injection. Scale bars, 1 mm (A–D), 100 mm (E, F).