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Fig. 2. Tubulin mutations perturb sidedness of asymmetric gene expression in Xenopus. Embryos injected with either tub4a mutant or Tubgcp2 mutant were processed for in situ hybridization at stage 22 with an Xnr-1 probe. (A) Both tubulin mutants deviated significantly (denoted with double asterisks) from control embryos (Tub4a: 64.7% incorrect expression, n = 51, P < <0.01 Welch’s t test; Tubgcp2: 32.5% incorrect expression, n = 83; control: 7.33% incorrect expression, n = 150, P < <0.01 Welch’s t test). (B–D) Xnr-1 expression pattern (purple stain) characterized in tubulin mutant mRNA-injected embryos. (B) Left expression indicated by one red arrow and one white arrow. (C) Absence of expression as indicated by two white arrows. (D) Bilateral expression as indicated by two red arrows. |
