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Fig. 2. Dorsoanterior views of the overlapping expression of noggin and cpl-1 in stage 13 embryos. All embryos were processed together for double in situ hybridization; however, the brown-staining substrate was omitted from the embryos in A, and the blue substrate was omitted from the embryos in B. (A) noggin expression (blue), at the anterior edge of the neural plate, and in the prechordal plate and notochord. (B) cpl-1 expression (brown), also in the anterior neural folds. (C) An embryo double stained for noggin and cpl-1, showing overlapping expression in the anterior neural folds. (The two stains together appear bluish-brown; compare with the blue noggin- expressing notochord). (D) Higher magnification view of C. Scale bars in A and D represent 0.5 mm. B and C are the same magnification as A. |
