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Fig. 1. Xmsx-1 and neural-inducing genes antagonized mutual expression. (A,B) The expression of noggin in a control uninjected
stage 11 embryo (A) and an embryo injected with Xmsx-1 ribonucleic acid (RNA; 5 ng/embryo) in two dorsal blastomeres at the
4-cell stage (B). Fixed embryos were processed for whole-mount in situ hybridization analysis. Note that the expression of endogenous
noggin at the gastrula stage was completely suppressed by the misexpression of Xmsx-1. (C,D) The expression of Xmsx-1 in animal
cap explants. Two-cell stage embryos were injected with noggin (C) or cerberus (Xcer, (D)) RNA, and the animal caps were excised
at stage 10 for reverse transcription–polymerase chain reaction (RT-PCR) analysis to detect Xmsx-1 expression. In animal caps, the
expression of Xmsx-1 was downregulated by the introduction of neural-inducing agents. |
