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Figure 3. The involvement of FGF signaling in organizer and paraxial mesoderm development. Embryos were treated with either DMSO or SU5402 from the 8- to 16-cell stage and analyzed at the gastrula stage for expression of the indicated transcripts. A,B,E-H,M-P: Embryos are displayed in a lateral blastoporal view. C,D,I-L,Q,R: Embryos are displayed from a lateral view with blastopore toward the bottom. Axial/organizer genes are present, but expression of paraxial mesodermal transcripts is absent in inhibitor-treated embryos. |
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Figure 4. Early FGF signaling is necessary to maintain organizer gene expression. Embryos were treated with either DMSO or SU5402 and analyzed at the early neurula stage for expression of a range of mesodermal, posterior and anterior, and endodermal genes, except for Q-V, which were analyzed at stage 28. A-H,W-ZD: Dorsal views with anterior to the left. I-M: Anterior views. O: Posterior view. J,L,N,P: Dorsal blastoporal views. Q-U: Lateral views, anterior to the left. V: Dorsal view. A-P: Embryos were treated from the 8- to 16-cell stage until the early neurula stage. R: Injected with XFD RNA at 250 pg into each blastomere at the 4-cell stage. S,U,V: Treated from the 8- to 16-cell stage until stage 28. W-ZD: Embryos were treated from mid-gastrulation until the early neurula stage. Y',Z': Embryos demonstrating the range of effects from inhibitor treatment. |
